pet22b vector backbone Search Results


mprod hspr pet22b vector  (New England Biolabs)
96
New England Biolabs mprod hspr pet22b vector
Mprod Hspr Pet22b Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mprod hspr pet22b vector/product/New England Biolabs
Average 96 stars, based on 1 article reviews
mprod hspr pet22b vector - by Bioz Stars, 2026-05
96/100 stars
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pet22b vector backbone  (GenScript corporation)
90
GenScript corporation pet22b vector backbone
Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in <t>pET22b</t> vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
Pet22b Vector Backbone, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet22b vector backbone/product/GenScript corporation
Average 90 stars, based on 1 article reviews
pet22b vector backbone - by Bioz Stars, 2026-05
90/100 stars
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pet22b pa wt  (Addgene inc)
92
Addgene inc pet22b pa wt
Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in <t>pET22b</t> vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
Pet22b Pa Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet22b pa wt/product/Addgene inc
Average 92 stars, based on 1 article reviews
pet22b pa wt - by Bioz Stars, 2026-05
92/100 stars
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pza teto egfp vector  (New England Biolabs)
99
New England Biolabs pza teto egfp vector
Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in <t>pET22b</t> vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
Pza Teto Egfp Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pza teto egfp vector/product/New England Biolabs
Average 99 stars, based on 1 article reviews
pza teto egfp vector - by Bioz Stars, 2026-05
99/100 stars
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q5 high-fidelity dna polymerase  (New England Biolabs)
99
New England Biolabs q5 high-fidelity dna polymerase
Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in <t>pET22b</t> vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
Q5 High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/q5 high-fidelity dna polymerase/product/New England Biolabs
Average 99 stars, based on 1 article reviews
q5 high-fidelity dna polymerase - by Bioz Stars, 2026-05
99/100 stars
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phusion high-fidelity pcr master mix with hf buffer  (New England Biolabs)
99
New England Biolabs phusion high-fidelity pcr master mix with hf buffer
Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in <t>pET22b</t> vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
Phusion High Fidelity Pcr Master Mix With Hf Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phusion high-fidelity pcr master mix with hf buffer/product/New England Biolabs
Average 99 stars, based on 1 article reviews
phusion high-fidelity pcr master mix with hf buffer - by Bioz Stars, 2026-05
99/100 stars
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t7 coupled reticulocyte lysate system  (Promega)
90
Promega t7 coupled reticulocyte lysate system
Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in <t>pET22b</t> vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
T7 Coupled Reticulocyte Lysate System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t7 coupled reticulocyte lysate system/product/Promega
Average 90 stars, based on 1 article reviews
t7 coupled reticulocyte lysate system - by Bioz Stars, 2026-05
90/100 stars
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hindiii  (New England Biolabs)
99
New England Biolabs hindiii
Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in <t>pET22b</t> vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
Hindiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hindiii/product/New England Biolabs
Average 99 stars, based on 1 article reviews
hindiii - by Bioz Stars, 2026-05
99/100 stars
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ncoi  (New England Biolabs)
96
New England Biolabs ncoi
Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in <t>pET22b</t> vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
Ncoi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ncoi/product/New England Biolabs
Average 96 stars, based on 1 article reviews
ncoi - by Bioz Stars, 2026-05
96/100 stars
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bmal1 tad  (Azenta)
86
Azenta bmal1 tad
Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in <t>pET22b</t> vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
Bmal1 Tad, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bmal1 tad/product/Azenta
Average 86 stars, based on 1 article reviews
bmal1 tad - by Bioz Stars, 2026-05
86/100 stars
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Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.

Journal: Frontiers in Microbiology

Article Title: Repurposing of FDA Approved Drugs Against SARS-CoV-2 Papain-Like Protease: Computational, Biochemical, and in vitro Studies

doi: 10.3389/fmicb.2022.877813

Figure Lengend Snippet: Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.

Article Snippet: The cDNA sequence coding for SCoV-2 PLpro was codon-optimised for bacterial expression and was custom-synthesised in pET22b vector backbone by GeneScript, USA.

Techniques: Plasmid Preparation, Expressing, Synthesized, Purification